Read our latest publication in the American Journal of Hematology
Erythropoietin downregulates red blood cell clearance, increasing transfusion efficacy in severely anemic recipients
The spleen, a major immune organ, contributes to the circulation of red blood cells, a process essential for survival. Rigid or adherent red blood cells are retained and eliminated by the spleen to prevent inadequate blood flow in other organs. The human spleen controls the quality of red blood cells by stringently challenging their deformability.
We study the circulatory capacity of red blood cells with reference tools (histology, cell morphology, ektacytometry, interference contrast, electron microscopy, mouse model, etc.) and with innovative methods (filtration by microbeads, flow imaging, ex-vivo perfusion of human spleens, splenomimetic microfluidics …). We have defined the splenic filtering functional unit, the splenon, by analogy with the renal filtering unit, the nephron. We also study the spleen-specific pitting process of pitting, by which rigid bodies are expelled from the red blood cell that returns to the circulation almost intact.
Concepts and applications are explored and envisioned in malaria, transfusion and red cell diseases.
Erythropoietin downregulates red blood cell clearance, increasing transfusion efficacy in severely anemic recipients
American journal of hematology, 2023, vol., p.
Casimir Madeleine, Colard Martin, Dussiot Michael, Roussel Camille, Martinez Anaïs, Peyssonnaux Carole, Mayeux Patrick, Benghiat Samantha, Manceau Sandra, Francois Anne, Marin Nathalie, Pène Frédéric, Buffet Pierre A, Hermine Olivier, Amireault Pascal
Safe drugs with high potential to block malaria transmission revealed by a spleen-mimetic screening
Nature communications, 2023, vol.14, p.1951
Mario Carucci, Julien Duez, Joel Tarning, Irene García-Barbazán, Aurélie Fricot-Monsinjon, Abdoulaye Sissoko, Lucie Dumas, Pablo Gamallo, Babette Beher, Pascal Amireault, Michael Dussiot, Ming Dao, Mitchell V Hull , Case W McNamara, Camille Roussel, Papa Alioune Ndour, Laura Maria Sanz, Francisco Javier Gamo, Pierre Buffet
Storage-Induced Micro-Erythrocytes Can Be Quantified and Sorted by Flow Cytometry
Frontiers in physiology, 2022, vol.13, p.838138
Marin Mickaël, Peltier Sandy, Hadjou Youcef, Georgeault Sonia, Dussiot Michaël, Roussel Camille, Hermine Olivier, Roingeard Philippe, Buffet Pierre A, Amireault Pascal
Splenic clearance of rigid erythrocytes as an inherited mechanism for splenomegaly and natural resistance to malaria
eBioMedicine, 2022, vol.82, p.104167
Henry B, Volle G, Akpovi H, Gineau L, Roussel C, Ndour PA, Tossou F, Suarez F, Palstra F, Fricot A, Chambrion C, Solinc J, Nguyen J, Garé M, Aussenac F, Cottart CH, Keyser C, Adamou R, Tichit M, Hardy D, Fievet N, Clain J, Garcia A, Courtin D, Hermine O, Sabbagh A, Buffet P
Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria
Frontiers in physiology, 2022, vol.13, p.875189
Chambrion C, Depond M, Angella L, Mouri O, Kendjo E, Fricot-Monsinjon A, Roussel C, Biligui S, Tantaoui I, Taieb A, Argy N, Houzé S, Piarroux R, Siriez JY, Jaureguiberry S, Larréché S, Théllier M, Cenac N, Buffet P, Ndour PA
Erythrocytic vacuoles that accumulate a fluorescent dye predict spleen size and function in sickle cell disease
American journal of hematology, 2022, vol.97, p.E385—E388
Sissoko A, Fricot-Monsinjon A, Roussel C, Manceau S, Dumas L, Capito C, Allali S, Yekkache N, Dussiot M, Nguyen Y, Lefort Des Ylouses A, Aussilhou B, Tichit M, Hardy D, Maître B, Eckly A, De Montalembert M, Cavazzana M, Joseph L, Buffet P
Intravenous Artesunate for the Treatment of Severe Imported Malaria: Implementation, Efficacy, and Safety in 1391 Patients.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2021, vol.73, p.
Roussel Camille, Ndour Papa Alioune, Kendjo Eric, Larréché Sébastien, Taieb Aida, Henry Benoît, Lebrun-Vignes Bénédicte, Chambrion Charlotte, Argy Nicolas, Houzé Sandrine, Mouri Oussama, Courtin David, Angoulvant Adela, Delacour Hervé, Gay Frédérick, Siriez Jean-Yves, Danis Martin, Bruneel Fabrice, Bouchaud Olivier, Caumes Eric, Piarroux Renaud, Thellier Marc, Jauréguiberry Stéphane, Buffet Pierre
Rapid clearance of storage-induced microerythrocytes alters transfusion recovery.
Blood, 2021, vol.137, p.
Roussel Camille*, Morel Alexandre*, Dussiot Michaël*, Marin Mickaël, Colard Martin, Fricot-Monsinjon Aurélie, Martinez Anaïs, Chambrion Charlotte, Henry Benoît, Casimir Madeleine, Volle Geoffroy, Dépond Mallorie, Dokmak Safi, Paye François, Sauvanet Alain, Le Van Kim Caroline, Colin Yves, Georgeault Sonia, Roingeard Philippe, Spitalnik Steven L, Ndour Papa Alioune, Hermine Olivier, Hod Eldad A, Buffet Pierre A**, Amireault Pascal**
Evaluation of splenic accumulation and colocalization of immature reticulocytes and Plasmodium vivax in asymptomatic malaria: A prospective human splenectomy study.
PLoS medicine, 2021, vol.18, p.
Kho Steven, Qotrunnada Labibah, Leonardo Leo, Andries Benediktus, Wardani Putu A I, Fricot Aurelie, Henry Benoit, Hardy David, Margyaningsih Nur I, Apriyanti Dwi, Puspitasari Agatha M, Prayoga Pak, Trianty Leily, Kenangalem Enny, Chretien Fabrice, Brousse Valentine, Safeukui Innocent, Del Portillo Hernando A, Fernandez-Becerra Carmen, Meibalan Elamaran, Marti Matthias, Price Ric N, Woodberry Tonia, Ndour Papa A, Russell Bruce M, Yeo Tsin W, Minigo Gabriela, Noviyanti Rintis, Poespoprodjo Jeanne R, Siregar Nurjati C, Buffet Pierre A*, Anstey Nicholas M*
Hidden Biomass of Intact Malaria Parasites in the Human Spleen.
The New England journal of medicine, 2021, vol.384, p.
Kho Steven, Qotrunnada Labibah, Leonardo Leo, Andries Benediktus, Wardani Putu A I, Fricot Aurelie, Henry Benoit, Hardy David, Margyaningsih Nur I, Apriyanti Dwi, Puspitasari Agatha M, Prayoga Pak, Trianty Leily, Kenangalem Enny, Chretien Fabrice, Safeukui Innocent, Del Portillo Hernando A, Fernandez-Becerra Carmen, Meibalan Elamaran, Marti Matthias, Price Ric N, Woodberry Tonia, Ndour Papa A, Russell Bruce M, Yeo Tsin W, Minigo Gabriela, Noviyanti Rintis, Poespoprodjo Jeanne R, Siregar Nurjati C, Buffet Pierre A*, Anstey Nicholas M*
Metabolic rejuvenation upgrades circulatory functions of red blood cells stored under blood bank conditions.
Transfusion, 2021, vol.61, p.
Marin Mickaël, Roussel Camille, Dussiot Michael, Ndour Papa A, Hermine Olivier, Colin Yves, Gray Alan, Landrigan Matt, Le Van Kim Caroline, Buffet Pierre A, Amireault Pascal
Address
Biotigr Lab
Team 4 UMR-S 1134 INSERM
Université de Paris
Hôpital NECKER – Enfants Malades
149 rue de Sèvres
75015 Paris, France
Contact Information
pierre.buffet@inserm.fr
mickael.marin@inserm.fr
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