Post-Doc, Spleen function in physiology and disease group

abdoulaye.sissoko@inserm.fr

Sissoko obtained his master degree in cell and molecular biology “specialized in parasitology” at Pierre and Marie Curie university, Paris. He had the chance to realize a six months internship on Saccharomyces cerevisiae as a model of cerebral disorder, the polymicrogyria which is related to human tubulin beta mutation. He focused on reproducing the F265L mutation on S. cerevisiae tubulin beta and undertook a functional study. Those results led to an original paper as co-author in Biology Open (2019).

Sissoko has then pursued with PhD thesis (2015-2018) in which he focused on elucidating artemisinins mechanism of action and targets in Plasmodium falciparum (Paris Descartes). He synthesized original fluorescent artemisinin-based probes which are the most similar to artemisinin derivatives, as compared to published artemisinin-probes so far. The obtained results led to three papers in 2020 as first author (one in ACS Infectious Diseases) and co-author (Communications Biology and BBA-Molecular Cell Research).

He joined the BioTiGr team in December 2019 as a postdoc fellow. He first worked for 1 year on validating malaria transmission blocking drugs in-vitro and in-vivo. Since February 2021, he is now focused on spleen function and markers in hyposlpenic, asplenic and splenectomized subjects.

Publications and patents

1. A. Sissoko, et al. Clearance of pathogenic erythrocytes is maintained despite spleen dysfunction in children with
sickle cell disease. American Journal of Hematology, 2024. DOI: 10.1002/AJH.27481

2. A. Sissoko, et al. Splenic filtration of red blood cells in physiology, malaria and sickle cell disease. Current Opinion in Hematology, 2024. DOI: 10.1097/MOH.0000000000000839

3. S. Kho, N C. Siregar, L. Qotrunnada, A. Fricot-Monsinjon, A. Sissoko, et al. Retention of uninfected red blood cells causing congestive splenomegaly is the major mechanism of anemia in malaria. American Journal of Hematology, 2023. DOI: 10.1002/ajh.27152

4. A. Sissoko, A. Fricot-Monsinjon, C. Roussel, S. Manceau, L. Dumas, C. Capito, S. Allali, N. Yekkache, M. Dussiot, Y. Nguyen, A. Lefort Des Ylouses, B. Aussilhou, M. Tichit, D. Hardy, B. Maître, A. Eckly, M. De Montalembert, M. Cavazzana, L. Joseph, P. Buffet. Erythrocytic vacuoles that accumulate a fluorescent dye predict spleen size and function in sickle cell disease. American Journal of Hematology, 2022. DOI: 10.1002/ajh.26690

5. M. Carucci, J. Duez, J. Tarning, I. García-Barbazán, A. Fricot-Monsinjon, A. Sissoko, L. Dumas, P. Gamallo, B. Beher, P. Amireault, M. Dussiot, M. Dao, M. Hull, C. McNamara, C. Roussel, PA. Ndour, LM. Sanz, FJ. Gamo, P. Buffet. Safe drugs to block the transmission of malaria revealed by a spleen-mimetic screening approach. Nature communications, 2022 (accepté dans l’attente de dernières modifications)

6. Y. Qiang, A. Sissoko, Z L. Liu, T. Dong, F. Zheng, F. Kong, J M. Higgins, G E. Karniadakis, P A. Buffet*, Subra Suresh*, Ming Dao*. Microfluidic study of retention and elimination of abnormal red blood cells by human spleen with implications for sickle cell disease. Proceedings of the National Academy of Sciences, 2022. https://doi.org/10.1073/pnas.2217607120

7. A. Sissoko, P. Vásquez-Ocmín, A. Maciuk, G. Neveu, D. Barbieri, R. Grougnet, M. Blaud, S. Michel, C. Lavazec, J. Clain, S. Houzé and R. Duval. A chemically-stable fluorescent mimic of artemether and arteether with conserved biological activity and specificity shows high mechanistic relevance to the clinical antimalarial drugs. ACS Infectious Diseases, 2020. DOI: 10.1021/acsinfecdis.9b00430

8. G. Bouyer, D. Barbieri, F. Dupuy, A. Marteau, A. Sissoko, M-E. N’Dri, G. Neveu, L. Bedault, D. Roman, G. Siciliano, P. Alano, R M. Martins, J-J. Lopez-Rubio, J. Clain, R. Duval, S. Egée and C. Lavazec. Activation of erythrocyte permeability enhances drug uptake by malaria sexual parasites. Communications Biology, 2020. DOI: 10.1038/s42003-020-01454-7

9. A. Laleve, C. Panozzo, I. Kühl, A. Bourand-Plantefol, J. Ostojic, A. Sissoko, D. Tribouillard-Tanvier, D. Cornu, A. Burg, B. Meunier, M. Blondel, J. Clain, N. Bonnefoy, R. Duval and G. Dujardin. Artemisinin and its derivatives early target mitochondrial cytochromes in yeast and human cells. BBA-Molecular Cell Research, 2020. DOI:10.1016/j.bbamcr.2020.118661

10. E. Denarier, C. Brousse, A. Sissoko, A. Andrieux and C. Boscheron. A neurodevelopmental TUBB2B β-tubulin mutation impairs Bim1 (yeast EB1)-dependent spindle positioning. Biology Open, 2019. DOI: 10.1242/bio.038620

11. Patent (BIO20390, Inserm transfert, 2022): automated fluorescence-based quantification of pocked red cells

12. Patent (BIO23561, Inserm transfert, 2024): methods of blocking transmission of Plasmodium falciparum