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Storage-Induced Micro-Erythrocytes Can Be Quantified and Sorted by Flow Cytometry
Small RBC (type III echinocytes, sphero-echinocytes, and spherocytes) defined as storage-induced micro-erythrocytes (SME) appear during storage in blood banks. SME accumulate with variable intensity from donor to donor, are cleared rapidly after transfusion, and thus provide a quantitative marker of storage quality. Here we describe a staining protocol that enables counting and sorting of SME using a standard flow cytometer. Marin et al.,2022
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Biotigr Lab
Team 4 UMR-S 1134 INSERM
Université de Paris
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75014 Paris, France
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pierre.buffet@inserm.fr
aurelie.fricot@inserm.fr
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