Storage-Induced Micro-Erythrocytes Can Be Quantified and Sorted by Flow Cytometry

Small RBC (type III echinocytes, sphero-echinocytes, and spherocytes) defined as storage-induced micro-erythrocytes (SME) appear during storage in blood banks. SME accumulate with variable intensity from donor to donor, are cleared rapidly after transfusion, and thus provide a quantitative marker of storage quality. Here we describe a staining protocol that enables counting and sorting of SME using a standard flow cytometer. Marin et al.,2022

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